玻璃化冷冻系统 - 韦拓生物 - 深圳韦拓生物科技有限公司

玻化冻存管由 Gábor Vajta 教授于 1998 年发明的一种专为玻璃化冷冻设计的开放式冷冻载体，内含保护管，可以有效防止液氮储存中的交叉污染。冷冻速率可达 20000°C/min (Vajta et al., 1998), 被认为是快速的玻璃化冷冻方法之一。玻化冻存管由冻存管和保护管组成，一盒五种颜色，可适用于卵子和各个时期胚胎的冷冻保存。

科研成果：

人卵母细胞玻璃化冷冻后出生婴儿 (Kuleshova et al., 1999)

胚胎玻璃化冷冻后的克隆动物研究 (Tecirlioglu et al., 2003)

未成熟卵母细胞玻璃化冷冻后出生牛犊 (Vieira et al., 2002)

人胚胎干细胞玻璃化冷冻后存活及发育率研究 (Reubinoff et al., 2001)

玻璃化冷冻、复苏卵母细胞及不同时期胚胎

此视频由韦拓生物首席科学家Gábor Vajta演示

参考文献

1. Vajta G., Holm P., Kumayama M., Booth P.J., Jacobsen H., Greve T., Callesen H. Open Pulled Straw (OPS) Vitrification: A New Way to Reduce Cryoinjuries of Bovine Ova and Embryos. Molecular Reproduction and Development. 1999; 51(1): 53-58. DOI: 10.1002/(sici)1098-2795(199809)51:1<53::aid-mrd6>3.0.co;2-v.

2. Kuleshova, L., Gianaroli, L., Magli, C., Ferraretti, A., Trounson, A. Birth following vitrification of a small number of human oocytes: Case Report. Human Reproduction. 1999; 14(12): 3077–3079. DOI:10.1093/humrep/14.12.3077.

3. Vieira, A., Mezzalira, A., Barbieri, D., Lehmkuhl, R., Rubin, M. I., Vajta, G. Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology. 2002; 45(1): 91–94. DOI: 10.1016/s0011-2240(02)00109-8.

4. Reubinoff, B. E., Pera, M. F., Vajta, G., Trounson, A. O. Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Human Reproduction. 2001; 16(10): 2187–2194. DOI:10.1093/humrep/16.10.2187.

5. Vajta G. Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reproductive Bio-Medicine Online. 2006; 12: 779-796. DOI: 10.1016/s1472-6483(10)61091-7.

6. Vajta G. Vitrification in human and domestic animal embryology: work in progress. Reproduction, Fertility and Development. 2012; 25(5): 719-727. DOI: 10.1071/RD12118.

7. Vajta G., Reichart A., Baldi F. U., Rienzi L. From a back up technology to a strategy-outlining approach: the success story of cryopreservation. Expert Rev. Obstet. Gynecol. 2013; 8(2): 181-190. DOI: 10.1586/eog.12.80.

8. Munck N. D, Vajta G. Safety and efficiency of oocyte vitrification, Cryobiology. 2017; 78: 119-127. DOI: 10.1016/j.cryobi-ol.2017.07.009.

9. Vajta G. Vitrification in ART: past, present and future. Theriogenology. 2020; 150: 276-279. DOI:1016/j.theriogenolo-gy.2020.01.057.

订购信息

产品	货号	规格
玻化冻存管(OPS)	V001001	红色x1、绿色x1、蓝色x1、黑色x1、透明x1
玻璃化冷冻液	V002001 V002002	基础液 1.0mL x1 冷冻液1 1.0mL x1 冷冻液2 1.0mL x1 基础液 4.5mL x1冷冻液1 4.5mL x1 冷冻液2 4.5mL x2
玻璃化解冻液	V003001 V003002	基础液 1.8mL x1 解冻液1 1.5mL x2 解冻液2 1.0mL x1 基础液 4.5mL x2解冻液1 4.5mL x1解冻液2 4.5mL x1
牙钳	VOPS01	___
热封钳	VOPS02	___
封口剪	VOPS03	___

质量控制

玻化冻存管（OPS)

内毒素 < 0.5EU/套

鼠胚囊胚扩张率 ≥ 80%

无菌测试：通过

玻璃化冷冻、解冻试剂

pH值 7.2-7.6

内毒素 < 0.25EU/mL

鼠胚囊胚扩张率 ≥ 80%

无菌测试：通过